Poly(lactic-co-glycolic acid) ..
Sigma-Aldrich Online Catalog Product List: PEG-PLGA
Synthesis, Characterization, and Study of PLGA …
Form last decade, researchers have worked towards biocompatibility, and controlled drug release to improve methodology of treatment of diseases. With improvement in Nano-micro technology, researchers have developed dual functional implantable devices which work as drug reservoir and release machine [1-2] on other hand, diffusion based drug release systems have got special attention because of its cost effective and simple synthesis methodology. Among various biocompatible polymers, PLGA poly (lactic-co-glycolic acid) is attracted noteworthy attention due to its attractive properties:
In the current report, we present a highly complex and multifunctional hybrid polymer lipid NP platform that incorporates diagnostic nanocrystals and two therapeutic drugs. The complexity of the proposed NP required selecting a suitable synthesis method that would facilitate the integration of all functionalities into the NP, since other widely used NP synthesis techniques, including nanoprecipitation, have failed at the incorporation of all necessary components into a NP. To this aim we used a two pronged approach that involved chemical modification of a polymer and the use of microfluidic technology, that we recently developed for high throughput polymer NP synthesis. This microfluidic system provides a controlled mixing environment, which facilitates NP assembly through microvortices at ambient conditions.
Formulation of functionalized PLGA polymeric nanoparticles ..
In summary, we combined simple carbodiimide conjugation chemistry to modify the PLGA polymer to synthesize a complex and multifunctional theranostic polymer lipid NP platform with microfluidic technology. Two drugs, i.e. the anti angiogenic drug SRF and cytotoxic drug DOX, for combined cancer therapy were successfully encapsulated in the lipid corona and polymeric core, respectively. Release profiles showed a quick release of SRF and a delayed release of DOX. Finally, an in vivo pilot NIRF imaging experiment revealed the accumulation of the NPs at the tumor site and immuno fluorescence of tumor sections revealed distribution of the NPs within the tumor space. Future studies are planned to evaluate the efficacy of our approach in vivo in tumor bearing mice. In addition to cancer therapy the flexibility of our NP platform allows for incorporation of other therapeutics and applications of a theranostic NP probe for atherosclerotic disease or other pathologies that are characterized by ongoing angiogenesis.
The polymer lipid NPs were assembled by dual microvortices created at the intersection of the three inlets of the microfluidic chip () at a flow rate of 5 ml/min in the external channels and 1 ml/min in the center channel, which corresponded to a Reynolds number of 150. These conditions provide controlled mixing environment on the microscale and facilitate the swift assembly of uniform NPs, as explained in details previously. The DOX loading, established by HPLC, was 25.6% of the initial input value. This value is 19 times higher than DOX loading in polymer lipid NPs where PLGA was not modified with HP βCDs (1.3% of the initial input value). Our strategy resulted in 2 (molar %) times higher DOX encapsulation in PLGA NPs as compared to a previously published method where DOX was directly conjugated to the PLGA polymer to elevate its encapsulation into the NP core and the NPs were formed using an emulsion solvent diffusion method. The SRF encapsulation efficiency was established to be 66.0% of the initial input value.
Study of Thermal Degradation of PLGA, PLGA Nanospheres …
The modified PLGA AuNCs HP βCDs was incubated with DOX prior to NP synthesis to assure DOX HPβCDs complex formation. The microfluidic chip for the rapid polymer lipid NP synthesis is shown in (). PLGA modified with AuNCs HP βCDs DOX in acetonitrile was infused in the center channel of a microfluidic chip. PEGylated phospholipids (PEG DSPE) and 1,2 dipalmitoyl sn glycero 3 phosphocholine (DPPC) in a 7:3 ratio were combined with SRF in 30 % methanol in water and infused in the external channels. 0.3 mol % of PEG DSPE labeled with the NIRF Cy7 dye was added to the lipids.
The NP core is composed of a biodegradable poly(lactic co glycolic acid) (PLGA) polymer, functionalized with gold nanocrystals (AuNCs), which serve as scaffolds that are loaded with doxorubicin (DOX), a widely used cytostatic agent, . Since AuNCs are electron dense and exhibit good X ray attenuation properties the NP platform can be imaged by electron microscopy and computed tomography (CT), . The NP coating is comprised of ordinary phospholipids and PEGylated phospholipids at a 7/3 ratio, which by themselves form stable disks. The latter facilitates the formation of a lipid monolayer around the PLGA core. This lipid coating provides biocompatibility, while PEG reduces clearance by the mononuclear phagocyte system (MPS) and thereby elongates circulation half lives. We also used the lipidic coating to co encapsulate a second lipophilic drug sorafenib (SRF). SRF is an angiogenesis inhibitor with anti tumor activity. Lastly, the PEG coating was labeled with Cy7 fluorophores for near infrared fluorescence (NIRF) imaging.
Synthesis and characterization of PLGA nanoparticles
PLGA-PEG Nanoparticle Synthesis - ResearchGate
Synthesis of PLGA-based ..
08/01/2018 · PLGA-PEG Nanoparticle Synthesis
Synthesis, characterization and protein adsorption behaviors of PLGA/PEG di-block co-polymer blend films
I plan to synthesize a PLGA-PEG drug loaded nanoparticles using ..
Polymerization of a racemic mixture of L- and D-lactides usually leads to the synthesis of ..
The synthesis PLA, PGA, and PLGA can be ..
Subtle changes in molecular architecture can have a profound effect on degradation and drug release rates. For example, altering the polymer end groups can affect water uptake and degradation rate. Typical examples are shown below. Our scientists can help you determine the characteristics necessary for your application through developmental input and custom synthesis. Please to discuss your needs.
Polylactic-co-Glycolic Acid (PLGA) as Biodegradable …
PLGA: Poly Lactic-Co-Glycolic Acid; PEG: Polyethylene Glycol; FDA: Food and Drug Administration; Alg-PLGANP: Alginate-PLGA Nanoparticle; PDI: Polydispersity Index; TRF: Tocotrienol Rich Fraction; FA: Folic Acid; α: Alpha; ZP: Zeta Potential; SEM: Scanning Electron Microscopy; PXRD: Powder X ray Diffraction; DSC: Differential Scanning Calorimetry; FTIR: Fourier Transform Infrared Spectroscopy; nm: Nanometer; ESI-ms/MS: Electro spray Ionization Mass Spectrometry
Characterization, and Study of PLGA Copolymer in ..
If additional product optimization is required, we can provide developmental input and custom synthesis. A large percentage of our polymer business at DURECT is devoted to the development of polymers with customized characteristics.
PLGA-PEGs - NSP-Functional polymers and copolymers
PLGA [poly (lactic-co-glycolic acid)] is synthesized by means of ring-opening co-polymerization of two different monomers, the cyclic dimers (1,4-dioxane-2,5-diones) of glycolic acid and lactic acid. Polymers can be synthesized as either random or block copolymers thereby imparting additional polymer properties. During polymerization, successive monomeric units (of glycolic or lactic acid) are linked together in PLGA(Figure 1) X: number of units of lactic acid; y: number of units of glycolic acid. Lactic acid: glycolic acid composition of PLGA alters properties of PLGA and its applicability by ester linkages, thus yielding linear, aliphatic polyester as a product [2-3].
PLGA-PEGs is one of the most commonly used ..
As of now research fraternity had focused on formulating spherical PLGA nanoparticle. Considering the dire need of more controlled release, one can expects research in tuning size and shape of the PLGA nanoparticles for drug delivery applications. On other hand we can expect application of PLGA in theranostic material based therapy where PLGA or related materials linked biocompatible fluorescent material or fluorescent Nanomaterial for Imaging-Guided chemo or photo thermal combinatorial therapy. Considering the future potential application of PLGA materials, in this article we have reviewed advancement of PLGA research in the year 2015. In PubMed, we searched number of articles published form 01/01/2015 to 31/12/2015 on PLGA, and we found 937 articles. Researchers have demonstrated a patrol of applications ranging from drug delivery to in vivo imaging system, which indicates the direction of future research and its current progress .
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