Synthesis of Prussian Blue-Coated ..
The prussianblue existed on ;the surface of the nanoparticles when the concentrationis 200 μM and in excess with 1000 μM.
CHARACTERIZATION OF PRUSSIAN BLUE NANOPARTICLES …
Here, we describe the use of biodegradable Prussian blue nanoparticles (PBNPs) in combination with anti-CTLA-4 checkpoint blockade immunotherapy for the treatment of advanced cancers.
After successfully confirming that we could convert our own ferritin proteins that were produced from the parts we constructed ( ) into Prussian blue ferritin the next step was to evaluate how the design of our parts could potentially affect the reporter activity of our Prussian blue ferritin. Based on the spatial modelling performed by our team it was suggested that assembly of the ferritin nanoparticle with TALE proteins directly fused was highly unlikely. This is because the TALE proteins are significantly larger than the ferritin subunits. Their size would likely result in steric hindrance and prevent the assembly of the full ferritin protein. In order to test the predictions put forward by our modelling we ensured that our protein samples were balanced in order to have the same number ferritin cores in each sample. The catalytic activity of these proteins was then compared. From the data gathered we saw that the Prussian blue ferritin with fused coils (even if TALES are additionally bound to the ferritin via coils) was more effective as a reporter than having the TALE proteins directly fused to the ferritin nanoparticle (Figure 19). The results from this experiment suggest that the predictions made by our model were correct. Using however alleviates this issue as these coils are small and would not interfere in the ferritin self-assembly but can be used to attach our TALES to create the FerriTALE.
Synthesis and study of Prussian blue type nanoparticles …
Figure 4. Comparison image of commercial ferritin to Prussian blue ferritin after the synthesis reaction. The synthesis reaction took place over a 12 hour time period.
(a) Coupling c(RGDyK) peptide to Fe3O4 NPs. (b) Cell uptake of c(RGDyK)-MC-Fe3O4 NPs by U87MG, MCF-7 and U87MG + c(RGDyK) block. (c) MRI of the cross section of the U87MG tumors implanted in mice: (A) without NPs, (B) with the injection of 300 µg of c(RGDyK)-MC-Fe3O4 NPs, and (C) with the injection of c(RGDyK)-MC-Fe3O4 NPs and blocking dose of c(RGDyK); Prussian blue staining of U87MG tumors in the presence of (D) c(RGDyK)-MC-Fe3O4 NPs and (E) c(RGDyK)-MC-Fe3O4 NPs plus blocking dose of c(RGDyK). Reproduced with permission from Ref. . Copyright (2008) American Chemical Society.
Biodegradable Prussian blue nanoparticles for …
Prussian blue nanoparticles have been widely explored MRI contrast agents, which could be stabilized with albumin and loaded with ICG . The obtained nanoparticle could utilize Prussian blue for MRI and ICG for NIRF-imaging/phototherapy. The resulting MRI/NIRF-guided phototherapeutic nanoparticle exhibited significant tumor growth inhibition without tumor recurrence .
Tetrahydrofuran hydroperoxide mediated synthesis of Prussian blue nanoparticles: A study of their electrocatalytic activity and intrinsic peroxidase-like behavior.
we describe the use of biodegradable Prussian blue nanoparticles ..
Strategies for Preparing Albumin-based Nanoparticles …
Prussian blue nanoparticles operate as a new generation of photothermal ablation agents for cancer therapy.
Journal of Nanoscience and Nanotechnology
Magnetic Prussian blue nanoparticles for targeted photothermal therapy under magnetic resonance imaging guidance.
Multifunctional Iron Oxide Nanoparticles for …
Synthesis of magnetic Prussian blue nanoparticles and the fabrication of chemically modified electrode.
Regional Centre of Advanced Technologies and Materials
This summer we were able to successfully characterize Prussian blue ferritin as a reporter for our system as well as demonstrate that we could use our constructed parts to produce our own functional Prussian blue ferritin. This characterization included a full kinetic analysis, pH optimization, temperature optimization, and exploring the use of Prussian blue ferritin in a strip assay. In conjunction with our modelling efforts we also were able to evaluate the efficacy of different techniques of constructing our FerriTALE. Moving forward with the data gathered so far on ferritin we would like to further explore its application to our system. This includes fine-tuning the use of ferritin as a reporter for our system based off of the modelling performed. In order to increase the efficacy of the Prussian blue ferritin produced from our parts we would like to also explore different iron loading techniques for ferritin in order to ensure that the nanoparticle is fully saturated with iron before it undergoes the Prussian blue synthesis process. In the context of our prototype we would also like to explore different methods of applying our Prussian blue ferritin to our strips during experiments in order to see if we can reduce the minimum level of detection for our strip assay. We are excited to use ferritin in the context of our full system and to see how future iGEM teams can make use of ferritin.
Researche institute in Czech Republic focuses on nanotechnology
Synthesis and characterization of Prussian blue modified magnetite nanoparticles and its application to the electrocatalytic reduction of H2O2.
Spindle-like Mesoporous α-Fe2O3 Anode Material …
In situ immobilization of glucose oxidase in chitosan-gold nanoparticle hybrid film on Prussian blue modified electrode for high-sensitivity glucose detection.
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