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Oligonucleotide synthesis scale - Generi Biotech

Oligo DNAsynthesis

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GeneWorks - Custom Oligos - Synthesis Scale

For oligos of standard length (≤ 33 nucleotides) we guarantee a certain yield of DNA (OD260 applying to a 20-mer) to be delivered according to the respective scale of synthesis and mode of purification.

Guidelines for primer design for PCR; Synthesis Scale for ..

With over 20 years of experience in oligo synthesis, Metabion can provide you with a full range of DNA oligonucleotides and modifications of highest quality at competitive prices!

Oligo Synthesis (Primers & Probes) - inqaba biotec

We apply proprietary processes to offer Optimised Application Oligos and synthetic nucleic acids in the highest quality and standards.

Unless requested, oligos are synthesized without either 3´or 5´ phosphate. The 5´ and/or 3’-phosphate is available as a modification at additional charge.

Unless requested, oligos are synthesized without either 3´or 5´ phosphate. The 5´ and/or 3’-phosphate is available as a modification at additional charge.

Oligonucleotide synthesis - Wikipedia

Oligo DNA synthesis

The trityl-group is colorless when attached to a DNA base but it gives a characteristic orange color once removed. The intensity of this color can be measured by UV spectrophotometry and it is directly related to the number of trityl molecules present. Following the first coupling step, the amount of trityl released during deblocking is directly proportional to the amount of full-length oligo synthesized in the previous cycle. When the trityl is cleaved during the deblocking step, the resulting trityl cation is orange in color. The intensity of this color can be measure by UV spectrophotometry. By comparing the intensities of the trityl cation produced after the first and last coupling steps, one can calculate the average successful base coupling per cycle and hence the coupling efficiency.

The trityl-group is colorless when attached to a DNA base but it gives a characteristic orange color once removed. The intensity of this color can be measured by UV spectrophotometry and it is directly related to the number of trityl molecules present. Following the first coupling step, the amount of trityl released during deblocking is directly proportional to the amount of full-length oligo synthesized in the previous cycle. When the trityl is cleaved during the deblocking step, the resulting trityl cation is orange in color. The intensity of this color can be measure by UV spectrophotometry. By comparing the intensities of the trityl cation produced after the first and last coupling steps, one can calculate the average successful base coupling per cycle and hence the coupling efficiency.

We have successfully used our in-house oligos in more than 1,000,000 PCR and Sanger sequencing reactions and more than 600 Q-block synthesis
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  • Express Oligos - Now at 25 nmol synthesis scale!

    Large Scale Oligo Synthesis

  • Express Oligos - Now at 25 nmol synthesis scale

    The scale of the oligo synthesis describes the amount of the CPG material to start the synthesis.

  • Oligo Synthesis - Services - Eton Bioscience Inc.

    Oligonucleotide synthesis is the chemical synthesis of relatively short fragments of ..

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Frequently Asked Oligo Synthesis Questions ..

For "critical" applications like gene synthesis, subcloning, mutagenesis and alike, purification should be considered for oligos > 45 nucleotides.

DNA Oligos - DNA Oligo Synthesis ..

For "critical" applications like gene synthesis, subcloning, mutagenesis and alike, purification should be considered for oligos > 45 nucleotides.

from chemical oligonucleotides synthesis

OPC® is an abbreviation for “oligonucleotide purification cartridge”. This purification method consists of a reverse-phase (RP) chromatography, which separates complete, newly synthesized oligonucleotides from failure sequences, by-products, and other impurities. Specifically, the OPC® cartridge binds the dimithyltrityl (DMT) of the trityl-on oligonucleotide (complete, newly synthesized) from non-DMT bearing molecules. This techniques performs best for oligonucleotides up to 40 nucleotides in length.

Primer Synthesis Scales & Cost:

If it happens that an oligo does not pass our in-house quality control, it needs to be resynthesized. In those cases we have to apologize a 1-2 days increase of shipping time.

Oligonucleotide primers for cDNA synthesis (1 mg/ml)

Many of the modified amidites are less stable and do not couple as efficiently as the unmodified bases (even though longer coupling procedures may be used). Therefore, failure sequences are more abundant than in normal synthesis. Consequently, most of the modified oligos should be purified by to remove the more abundant failure sequences. Final yields are reduced as a result of the purification step, which results in a much purer final product, nearly 100% modified.

Custom Oligo Synthesis - Quintara Biosciences

Many of the modified amidites are less stable and do not couple as efficiently as the unmodified bases (even though longer coupling procedures may be used). Therefore, failure sequences are more abundant than in normal synthesis. Consequently, most of the modified oligos should be purified by to remove the more abundant failure sequences. Final yields are reduced as a result of the purification step, which results in a much purer final product, nearly 100% modified.

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