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Why is DNA replication performed in the 5' to 3 ..

One end of the RNA molecule is called the 5' (5-prime) end and the other the 3' (3-prime) end.

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That is 3' to 5' synthesis does not allow nucleotide ..


Rationale:
New unit: GENETICS
Need to know how DNA works to know how genetics work
Questions we will try to answer:
What is life?
What are inherited or genetic diseases
Why do I look the way I do?
How does my body grow?
DNA Structure
3 parts:
Sugar
Phosphate
Nitrogenous base
Quick Summary
Overview Replication
Overview: Transcription and Translation
Transciption and Translation Summary
Messenger RNA transcribes the message from the DNA
tRNA Facts
What are some proteins that the body makes?
But wait!!!
Before the mRNA can leave the nucleus it needs to be PROCESSED
Introns -- get cut out (stay IN the nucleus)
Exons -- EXIT the nucleus as part of finalized mRNA

4/23/2010 · What do we mean when we talk about the 3' and 5' ends of DNA or RNA

A major challenge in DNA replication arises from the fact that DNA polymerases cannot directly use a single strand of DNA as substrate. Instead, they require that a short stretch of complementary nucleotides, the primer, be in place before any new nucleotides can be added. The primer is generated by the pol-prim complex, composed of DNA primase, which synthesizes an eight to ten nucleotide stretch of complementary RNA on the single strand template, and DNA polymerase α, which extends the RNA by adding deoxyribonucleotides to a total length of about thirty bases. At this point, pol-prim dissociates from the template-primer, and DNA synthesis is continued by the primary replicative DNA polymerases.

Why is DNA replicated in the 5'-3' direction? - Quora

Why is DNA replicated in the 5'-3 ..


DNA is
always BUILT
or
READ
in the
5' - 3' DIRECTION.
Top of the strand is 3'.
Bottom of the strand is 5'.
3 prime to 5 prime
DNA is
always BUILT or READ
in the
5'- 3' DIRECTION
(like we read books from left to right)
This process continues along the DNA strands and forms
TWO
separate, IDENTICAL
DNA molecules
.
Each DNA molecule will have
one original strand
and
one new strand
(a.k.a
1 "parent" strand
and
1 "daughter" strand
).

Nucleotides
CAN'T
be added to its
5'
end because it would mean the DNA is being built in
the
3'
- 5' direction
(we don't read books from right to left)
.

What does prime stands for 5'-3' in DNA?

DNA synthesis occurs only in 5'-3' direction.?


So, the nucleotides/bases work in
threes
or
triplets
(the
CODON,
e.g
A U G
), resulting in 64 combinations :)
Where DNA contains a protein synthesis code,
mRNA's
version of it is a
CODON sequence
.
Translation occurs in
3 STEPS
:
INITIATION
,
ELONGATION
, and
TERMINATION
INITIATION
Once the mRNA arrives at a ribosome,
ribosomal RNA

(rRNA)
- which is the
RNA component of the ribosome
and essential for protein synthesis -
aligns
the strand into the correct position on the ribosome.

Nucleotides
CAN'T
be added to its
5'
end because it would mean the DNA is being built in
the
3'
- 5' direction
(we don't read books from right to left)
.

16/01/2018 · Why can't DNA polymerase attach things to the 5' end of a ..
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  • 28/11/2017 · DNA Replication: Prime ..

    5 DNA RNA Protein Synthesis 1

  • Primers used for dna synthesis : Primer (molecular …

    Post-DNA synthesis 5 hr M ..

  • RNA-DNA oligonucleotides that prime synthesis of new DNA

    05/08/1987 · Characterization of the DNA synthesis primed by T4 61 ..

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of the DNA double helix that is oriented in a 3' to 5' manner

A. comparing the amino acid sequence of proteins to the nucleotide sequence of their genes in humans
B. analyzing the sequence of RNAs produced from known DNA sequences
C. analyzing mutants that changed the code
D. examining the polypeptides produced when RNAs of known sequence were translatedAnswers seen: A (16), B (13), C (9), D (25)Explanation: This is the approach first used by Nirenberg.

Dna primer synthesis - Answers on HealthTap

Although the discovery of DNA dates back to 1866 when Gregor Mendel proved the inheritance of factors in pea plants, DNA testing is relatively new and have been the prime factor when solving crimes in general....

Directionality (molecular biology) - Wikipedia

A. analysis of mutants defective in DNA synthesis
B. study of human patients with defects in DNA repair
C. density gradient centrifugation of bacterial DNA sampled at different times after a shift from density-labeled medium to light medium
D. sucrose gradient centrifugation of bacterial DNA sampled at different times after a shift from normal medium to medium containing 32PAnswers seen: A (1), B (1), C (41), D (20)Explanation: This is the Meselson-Stahl experiment.

The 5′-end (pronounced "five prime ..

5' is the location of the
first deoxyribose sugar
.
RECAP 4 ->
DNA nucleotides are made up of:
SUGAR
+
PHOSPHATE
+
BASE

(A, G, C, T)
*In RNA, the sugar is ribose.
DNA HELICASE
breaks the helix open
The strands

are
OPPOSITES
of
each other.


- Bottom to top is 5' - 3'

or sugar to phosphate ->.

- Top to bottom is 3'- 5' or phosphate to sugar ->).
And because DNA is
anti-parallel
, the
5' - 3' strand
will always have nucleotides
added
to its

3' end
(the opposite of its 3' is
5'
).

5'
3'
5'
3'
5'
3'
original strand
new strand with added nucleotides
A
T
G
C
C
G
T
A
this new strand is being built in a
continuous straight line
in the
5' to 3' direction
.

Does DNA polymerase I require a $3

Inside cells, nucleic acid synthesis occurs by formation of new phosphodiester linkages at the 3’ end of a growing polymer. Although all biomolecule polymers are synthesized in only one direction, the 5’ to 3’ nature of nucleic acid polymers is of particular relevance to many cellular processes, including DNA replication, protein synthesis, and DNA damage repair. Understanding how DNA polymers form is vital to analyzing DNA replication and gene expression in living cells.

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