cDNA Synthesis Kits—SMART cDNA Synthesis
Clontech's SMART cDNA Synthesis Kit - Biocompare
cDNA Library Construction Kits - SMART cDNA Synthesis
"Like our other SMART-Seq v4 products, the SMART-Seq v4 Ultra Low Input RNA Kit for the Fluidigm C1 System provides higher sensitivity (a larger number of genes detected), more accurate expression data for genes with high GC content, and a higher yield of cDNA compared to existing chemistry," commented , Vice President of Research and Development at Clontech Laboratories, Inc. "This chemistry also produces higher consistency among replicates. This is important because the reduced technical variations will increase the likelihood of discovering true biological variations."
One of the greatest advantages of SMART technology is its increased efficiency compared to traditional technologies such as adaptor ligation. Its high efficiency and sensitivity enables you to use a very limited quantity of starting material, such as microdissected tissues, laser-captured cells, biopsy samples, etc. As little as 1-2 ng of total RNA is sufficient for generating a highly representative cDNA pool for different downstream applications.
of Clontech SMART-based kits for construction of cDNA ..
SMART (Switching Mechanism at 5’ End of RNA Template) is a unique technology that allows the efficient incorporation of known sequences at both ends of cDNA during first strand synthesis, without adaptor ligation. The presence of these known sequences is crucial for a number of downstream applications including amplification, RACE, and library construction. While a wide variety of technologies can be employed to take advantage of these known sequences, the simplicity and efficiency of the single-step SMART process permits unparalleled sensitivity and ensures that full-length cDNA is generated and amplified.
During first-strand SMARTer cDNA synthesis known universal primer sequences are incorporated at both ends of the cDNA. Following first-strand synthesis, SMARTer cDNA is immediately available for PCR amplification.
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Clontech Laboratories, Inc., a wholly owned subsidiary of Takara Bio Inc., develops, manufactures, and distributes a wide range of life science research reagents under the Takara, Clontech, and Cellartis brands. Key products include SMART cDNA synthesis kits for a variety of samples and applications, including NGS; high-performance qPCR and PCR reagents (including the Takara , Takara, Titanium, and Advantage enzymes); Cellartis stem cells and stem cell reagents; RT enzymes and SMART library construction kits; the innovative In-Fusion cloning system; Guide-it gene editing tools; Tet-based inducible gene expression systems; and Living Colors fluorescent proteins. These and other products support applications including NGS; gene discovery, regulation, and function studies; protein expression and purification; RNAi, gene editing, and stem cell studies; and plant and food research. For more information, visit .
and , /PRNewswire/ -- Clontech Laboratories, Inc., a wholly owned subsidiary of Takara Bio Inc., and Fluidigm Corporation today announced the recent launch of Clontech's . This kit, which is available for 4 and 10 IFCs (integrated fluidic circuits), allows high-quality cDNA synthesis starting from 96 single cells that have been isolated and processed with the Fluidigm C1 System, using .
(SMART) cDNA Synthesis kits for ..
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cDNA Synthesis Kits ..
SMARTer™ PCR cDNA Synthesis Kit :
SMARTerâ ¢ Pico PCR cDNA Synthesis Kit User Read more about cdna, synthesis, pico, tube, cycles and protocol.
SMART PCR cDNA Synthesis kit (Clontech…
the primers used are similar to those from the SMART cDNA synthesis kit to ..
RNA Kit allows quality cDNA synthesis starting ..
The SMART cDNA Library Construction Kit is designed for the cloning of full-length cDNA into a phage TriplEx2 vector. The kit combines the SMART technology (Switching Mechanism At 5' end of RNA Template) for cDNA amplification with adaptor-free, directional cloning into the TriplEx2 vector. This kit contains two separate protocols, allowing you to choose a method based on your starting material. The first protocol employs long-distance PCR (LD PCR), for researchers limited by their starting material. As little as 50 ng of total RNA can be used as starting material (1). The second protocol provides a more straightforward protocol for researchers with abundant amounts of starting material (i.e., 1 µg or more of poly A+ RNA). SMART libraries contain a higher percentage of full-length clones than libraries constructed by conventional methods or other full-length cDNA synthesis protocols. Thus, clones isolated from SMART cDNA libraries contain sequences corresponding to the complete 5' untranslated region of the mRNA (2).
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