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The biosynthesis of cell constituents I

6 While biosynthesis of most cell constituents occurs all through interphase from BIOLOGY 101 at Liberty

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Objectives Why is ATP a high-energy phosphate compound

AB - During investigations of fatty acid metabolism in Escherichia coli, the need arose for a method to differentiate between the incorporation of C14-labeled compounds into fatty acids and the incorporation into non-lipid cell constituents. Although a solvent extraction method (1) is available that yields quantitative and reproducible results for fatty acid biosynthesis, a somewhat simpler method was required which would allow rapid processing of a large number of samples. The method reported in this paper will allow rapid measurement of only that portion of the C14-labeled compound which is incorporated into fatty acids. The assay is not affected by incorporation of the labeled compound into other cellular constituents (e.g., protein). The method is based upon the solubility of fatty acids in hexane and the virtual insolubility of the other bacterial cell constituents in hexane.

High phosphoryl transfer potential compounds

N2 - During investigations of fatty acid metabolism in Escherichia coli, the need arose for a method to differentiate between the incorporation of C14-labeled compounds into fatty acids and the incorporation into non-lipid cell constituents. Although a solvent extraction method (1) is available that yields quantitative and reproducible results for fatty acid biosynthesis, a somewhat simpler method was required which would allow rapid processing of a large number of samples. The method reported in this paper will allow rapid measurement of only that portion of the C14-labeled compound which is incorporated into fatty acids. The assay is not affected by incorporation of the labeled compound into other cellular constituents (e.g., protein). The method is based upon the solubility of fatty acids in hexane and the virtual insolubility of the other bacterial cell constituents in hexane.

Biosynthesis of the Cell Walls of the Algae | SpringerLink

Biosynthesis of Cell Constituents From C 2-Compounds: Formation of Glycerate from Oxalate by Pseudomonas oxalaticus (OXI) grown in Oxalate…

During investigations of fatty acid metabolism in Escherichia coli, the need arose for a method to differentiate between the incorporation of C14-labeled compounds into fatty acids and the incorporation into non-lipid cell constituents. Although a solvent extraction method (1) is available that yields quantitative and reproducible results for fatty acid biosynthesis, a somewhat simpler method was required which would allow rapid processing of a large number of samples. The method reported in this paper will allow rapid measurement of only that portion of the C14-labeled compound which is incorporated into fatty acids. The assay is not affected by incorporation of the labeled compound into other cellular constituents (e.g., protein). The method is based upon the solubility of fatty acids in hexane and the virtual insolubility of the other bacterial cell constituents in hexane.

Influence of medium constituents on the biosynthesis …

Biosynthesis of Cell Constituents From C 2-Compounds: Formation of Glycerate from Oxalate by Pseudomonas oxalaticus (OXI) grown in Oxalate. J. …

The effect of different medium constituents for better yield of CPC ..
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